SH2D1A Polyclonal antibody

SH2D1A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11868-1-AP

Synonyms

DSHP, Duncan disease SH2 protein, EBVS, IMD5, LYP, MTCP1, SAP, SH2 domain protein 1A, SH2D1A, SLAM associated protein, XLP, XLPD

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Validation Data Gallery

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  WB analysis of mouse thymus using 11868-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11868-1-AP (SH2D1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse thymus tissue, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11868-1-AP targets SH2D1A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH2D1A fusion protein Ag2450
• Full Name: SH2 domain protein 1A
• Calculated Molecular Weight: 128 aa, 14 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC020732
• Gene Symbol: SH2D1A
• Gene ID (NCBI): 4068
• RRID: AB_2239283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH2D1A, also named as DSHP and SAP, is an inhibitor of the SLAM self-association. It acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. SH2D1A mediates interaction between FYN and SLAMF1. Defects in SH2D1A are a cause of lymphoproliferative syndrome X-linked type 1 (XLP1).

Publications

Species	Application	Title
human	WB	Arthritis Rheum; Decreased microRNA-142-3p/5p expression causes CD4+ T cell activation and B cell hyperstimulation in systemic lupus erythematosus.; Authors - Ding Shu S