SGLT2 Polyclonal antibody, PBS Only

SGLT2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28683-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 28683-1-AP (same clone as 28683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28683-1-AP (SGLT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

---

Product Information

28683-1-PBS targets SGLT2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGLT2 fusion protein Ag30120
• Full Name: solute carrier family 5 (sodium/glucose cotransporter), member 2
• Calculated Molecular Weight: 672 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC131542
• Gene Symbol: SGLT2
• Gene ID (NCBI): 6524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.