• Featured Product
  • KD/KO Validated

SGLT2 Polyclonal antibody

SGLT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 24654-1-AP

Synonyms

Na(+)/glucose cotransporter 2, SGLT2, SLC5A2, Sodium/glucose cotransporter 2



Tested Applications

Positive WB detected inmouse kidney tissue, A549 cells, 37°C incubated mouse kidney, rat kidney tissue
Positive IHC detected inhuman kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24654-1-AP targets SGLT2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGLT2 fusion protein Ag19163
Full Name solute carrier family 5 (sodium/glucose cotransporter), member 2
Calculated Molecular Weight 672 aa, 73 kDa
Observed Molecular Weight 69-70 kDa
GenBank Accession NumberBC131542
Gene Symbol SGLT2
Gene ID (NCBI) 6524
RRIDAB_2750601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.

Protocols

Product Specific Protocols
WB protocol for SGLT2 antibody 24654-1-APDownload protocol
IHC protocol for SGLT2 antibody 24654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Circulation

Sodium Glucose Cotransporter-2 Inhibitor Empagliflozin Reduces Infarct Size Independently of Sodium Glucose Cotransporter-2

Authors - Sha Chen
humanIHC

Cancer Lett

SGLT2 promotes pancreatic cancer progression by activating the Hippo signaling pathway via the hnRNPK-YAP1 axis.

Authors - Dianyun Ren
humanWB

Mol Metab

Sodium-glucose co-transporter2 expression and inflammatory activity in diabetic atherosclerotic plaques: Effects of sodium-glucose co-transporter2 inhibitor treatment.

Authors - Nunzia D'Onofrio
  • KD Validated
mouseIHC

Int J Mol Sci

Redefining the Role of ADAM17 in Renal Proximal Tubular Cells and Its Implications in an Obese Mouse Model of Pre-Diabetes.

Authors - Vanesa Palau
mouseIF

Metabolism

Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.

Authors - Hitomi Otomo
humanWB

Front Pharmacol

Empagliflozin Alleviates Hepatic Steatosis by Activating the AMPK-TET2-Autophagy Pathway in vivo and in vitro.

Authors - Ting Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Elina (Verified Customer) (03-04-2019)

The antibody is quite specific, used at a 1:200 dilution in mouse kidney. Comparison to a secondary only staining shows some background. Overall it appears to work well in paraffin embedded tissues, (5um thick).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse kidney
SGLT2 Antibody Immunofluorescence, validation (1:200 dilution) in mouse kidney (Cat no:24654-1-AP)