Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 30861-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 30861-1-AP
IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug.
IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug.
IHC staining of mouse small intestine using 30861-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 30861-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 30861-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse small intestine using 30861-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SGLT1 antibody (30861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SGLT1 antibody (30861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse colon tissue, mouse kidney tissue, mouse small intestine tissue, rat colon tissue
Positive IP detected in
mouse kidney tissue
Positive IHC detected in
mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse small intestine tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SLC5A1, also known as SGLT1, is a sodium-dependent glucose transporter (SGLT) family member. SGLT1 couples sugar transport to Na+ gradients across the intestinal brush border (PMID: 8563765). SGLT1 mediates active glucose and galactose absorption in the intestine and renal glucose scavenging. Mutations in SGLT1 cause glucose-galactose malabsorption (GGM) syndrome (PMID: 34880492). SGLT1 is expressed in intestine and endometrial cells (PMID: 2490366, 28974690).
Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism
Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 30861-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse small intestine using 30861-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 30861-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 30861-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse kidney using 30861-1-AP
IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug.
IF-P Figures
IF Staining of mouse small intestine using 30861-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SGLT1 antibody (30861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SGLT1 Polyclonal antibody
Catalog Number
30861-1-AP
Citations
2
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF-P : 1:200-1:800
Applications
WB, IHC, IF-P, IP, ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
