Tested Applications
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22100-1-AP targets SGK3 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17308 Product name: Recombinant human SGK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 371-496 aa of BC015326 Sequence: DNILHKPLSLRPGVSLTAWSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSEDLFL Predict reactive species |
| Full Name | serum/glucocorticoid regulated kinase family, member 3 |
| Calculated Molecular Weight | 496 aa, 57 kDa |
| Observed Molecular Weight | 42 kDa, 57 kDa |
| GenBank Accession Number | BC015326 |
| Gene Symbol | SGK3 |
| Gene ID (NCBI) | 23678 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53EW6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa and there is a isoform X3 can be detected with 378 amino acids(Sequence ID: ref|XP_005251262.1|).
