SGK196 Polyclonal antibody

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  WB analysis using 27444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27444-1-AP (SGK196 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SGK196 Polyclonal Antibody for WB, ELISA

Cat No. 27444-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

POMK, Sugen kinase 196, Protein O-mannose kinase

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27444-1-AP targets SGK196 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGK196 fusion protein Ag24922
• Full Name: protein kinase-like protein SgK196
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC101548
• Gene Symbol: SGK196
• Gene ID (NCBI): 84197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H5K3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SGK196, also known as POMK (protein O-mannose kinase), is an enzyme that plays a significant role in multiple biological processes. As a type II transmembrane protein, SGK196 consists of a cytoplasmic tail, one transmembrane helix, and a luminal domain (PMID: 31913260). It is widely expressed in tissues such as skeletal muscle, brain, heart, and kidney. SGK196 has been identified as a valuable marker for melanoma and has been found to suppress the metastasis of basal-like breast cancer cells, suggesting its role in tumorigenesis and cell survival.