SGCZ Polyclonal antibody

SGCZ Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 21614-1-AP

Synonyms

ZSG1, Zeta-SG, Zeta-sarcoglycan, Zeta sarcoglycan

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Validation Data Gallery

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  WB analysis using 21614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21614-1-AP (SGCZ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21614-1-AP (SGCZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse heart using 21614-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SGCZ antibody (21614-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse brain tissue, rat heart tissue
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21614-1-AP targets SGCZ in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGCZ fusion protein Ag16305
• Full Name: sarcoglycan zeta
• Calculated Molecular Weight: 265 aa, 30 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC125037
• Gene Symbol: SGCZ
• Gene ID (NCBI): 137868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sarcoglycan zeta, also known as SGCZ, ZSG1, is part of the sarcoglycan complex. The sarcoglycan complex is part of the dystrophin-associated glycoprotein complex (DGC), which bridges the inner cytoskeleton and the extracellular matrix. SGCZ plays a role in the maintenance of striated muscle membrane stability. SGCZ was also found as a component of the vascular smooth muscle sarcoglycan complex. SGCZ was reduced at the membrane in muscular dystrophy (PMID: 12189167).