SFRS5 Polyclonal antibody

SFRS5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 16237-1-AP

Synonyms

Delayed early protein HRS, HRS, Pre mRNA splicing factor SRP40, SFRS5, SRP40, SRSF5

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Validation Data Gallery

• 

  WB analysis of mouse testis using 16237-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16237-1-AP (SFRS5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16237-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16237-1-AP (SFRS5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

16237-1-AP targets SFRS5 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRS5 fusion protein Ag8988
• Full Name: splicing factor, arginine/serine-rich 5
• Calculated Molecular Weight: 272 aa, 31 kDa
• GenBank Accession Number: BC018823
• Gene Symbol: SFRS5
• Gene ID (NCBI): 6430
• RRID: AB_2185204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SR proteins own a characteristic C-terminal Ser/Arg-rich repeat (RS domain) of variable length and constitute a family of highly conserved nuclear phosphoproteins that can function as both essential and alternative pre-mRNA splicing factors [PMID:7556075]. The binding of SR proteins to pre-mRNA appears to be one of the earliest steps in spliceosome assembly, enhancing the binding of U1 snRNP to the 5' splice site to form a commitment complex [PMID:1577277 ]. Alternative splicing has a major role in the regulation of gene expression. SFRS5 belongs to the serine/arginine (SR) protein family of regulators of alternative pre-mRNA splicing [PMID:9244433].

Publications

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
human	WB	JCI Insight; Splicing factor SRSF6 mediates pleural fibrosis.; Authors - Li-Mei Liang
mouse		J Dent Res; Mutations Causing X-Linked Amelogenesis Imperfecta Alter miRNA Formation from Amelogenin Exon4; Authors - R Shemirani
mouse	WB	Cell Rep; Aspartyl-tRNA synthetase 2 orchestrates iron-sulfur metabolism in hematopoietic stem cells via fine-tuning alternative RNA splicing; Authors - Xuan Gu
human	IF	Molecules; L-Arginine Enhances Oral Keratinocyte Proliferation under High-Glucose Conditions via Upregulation of CYP1A1, SKP2, and SRSF5; Authors - Junhe Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yan (Verified Customer) (04-28-2021)

Signals was observed in the nucleus when I performed immunofluorescence.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Osteosarcoma Epithelial Cells