SRp20 Polyclonal antibody

SRp20 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10916-1-AP

Synonyms

Pre mRNA splicing factor SRP20, SFRS3, SRp20, SRSF3

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Validation Data Gallery

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  IHC staining of human cervical cancer using 10916-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10916-1-AP (SRp20 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 10916-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10916-1-AP (SRp20 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10916-1-AP (SRp20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human cervical cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

This antibody is recommended for IHC.

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

10916-1-AP targets SRp20 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRp20 fusion protein Ag1341
• Full Name: splicing factor, arginine/serine-rich 3
• Calculated Molecular Weight: 20 kDa
• GenBank Accession Number: BC000914
• Gene Symbol: SRSF3
• Gene ID (NCBI): 6428
• RRID: AB_2185064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS3, also named as SRSF3 and SRP20, belongs to the splicing factor SR family. It may be involved in RNA processing in relation with cellular proliferation and/or maturation. SFRS3 is one of the cognate factors. It is a proto-oncogene critical for cell proliferation and tumor induction and maintenance.The MW of SFRS3 is 20kd.

Publications

Species	Application	Title
human	WB,IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
		Mol Cell; Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition.; Authors - Miha Modic
human	IHC	Front Oncol; SRSF3 Expression Serves as a Potential Biomarker for Prognostic and Immune Response in Pan-Cancer.; Authors - Zihua Li
human	WB	Pathol Res Pract; LINC01210 promotes malignant phenotypes of colorectal cancer through epigenetically upregulating SRSF3.; Authors - Jia Luo
rat	WB	Rev Port Cardiol; Mesenchymal stem cells may alleviate angiotensin II-induced myocardial fibrosis and hypertrophy by upregulating SFRS3 expression; Authors - Ling Gu