ASF/SF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ASF/SF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

32

Reviews (2)

Cat no : 12929-2-AP

Synonyms

SFRS1, pre-mRNA-splicing factor SF2, P33 subunit, OK/SW-cl.3, ASF-1, ASF 1

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Validation Data Gallery

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  WB analysis using 12929-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12929-2-AP (ASF/SF2 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12929-2-AP

  IP result of anti-ASF/SF2 (IP:12929-2-AP, 3ug; Detection:12929-2-AP 1:500) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of human gliomas using 12929-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12929-2-AP (ASF/SF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12929-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12929-2-AP (ASF/SF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12929-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASF/SF2 antibody (12929-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:9000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 28 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below
• RIP: See 6 publications below

Product Information

12929-2-AP targets ASF/SF2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASF/SF2 fusion protein Ag3626
• Full Name: splicing factor, arginine/serine-rich 1
• Calculated Molecular Weight: 248 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC010264
• Gene Symbol: ASF/SF2
• Gene ID (NCBI): 6426
• RRID: AB_2187211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	WB	J Med Virol; TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1 expression; Authors - Lin Guo
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan
mouse,human	IF,WB	Cell Death Differ; The long non-coding RNA PFI protects against pulmonary fibrosis by interacting with splicing regulator SRSF1.; Authors - Jian Sun
human	WB,IF	Angiogenesis; Extracellular matrix stiffness controls VEGF165 secretion and neuroblastoma angiogenesis via the YAP/RUNX2/SRSF1 axis.; Authors - Min Bao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katie (Verified Customer) (03-14-2022)

Works well for western blot - clean blot with one band.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 Western 1:50 IF
• Cell Tissue Type: EL4 cells / Primary Murine CD4 T cells

FH

Yan (Verified Customer) (04-28-2021)

It worked very well when I performed immunofluorescence. Signal was observed in the nucleus.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Osteosarcoma Epithelial Cells