SFRP2 Polyclonal antibody

SFRP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12189-1-AP

Synonyms

sFRP-2, Secreted frizzled-related protein 2, Secreted apoptosis-related protein 1, SDF-5, SARP1

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Validation Data Gallery

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  WB analysis using 12189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12189-1-AP (SFRP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 12189-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SFRP2 antibody (12189-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: rat heart tissue, mouse heart tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

12189-1-AP targets SFRP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRP2 fusion protein Ag2853
• Full Name: secreted frizzled-related protein 2
• Calculated Molecular Weight: 295 aa, 34 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC008666
• Gene Symbol: SFRP2
• Gene ID (NCBI): 6423
• RRID: AB_2254520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

Publications

Species	Application	Title
human	WB	Cell Res; Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs.; Authors - Chenlin Zhou
human	WB	Front Oncol; Fucoidan Inhibits the Progression of Hepatocellular Carcinoma via Causing lncRNA LINC00261 Overexpression.; Authors - Danhui Ma
human	WB	Biomed Pharmacother; MicroRNA-500a promotes migration and invasion in hepatocellular carcinoma by activating the Wnt/β-catenin signaling pathway.; Authors - Yuntao Guo
mouse	WB	PLoS One; Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis.; Authors - Zhang Xiujun X
		Physiol Genomics; Tenotomy Immobilization as a Model to Investigate Skeletal Muscle Fibrosis (with Emphasis on Secreted Frizzled-Related Protein 2).; Authors - Uğur Akpulat
mouse	WB	Psychiatry Investig; m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis; Authors - Yue Ming