SFRP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SFRP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 26460-1-AP

Synonyms

FRP, FRP 1, FRP1, FrzA, SARP 2, SARP2, sFRP 1, SFRP1

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Validation Data Gallery

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  WB analysis using 26460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26460-1-AP (SFRP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26460-1-AP (SFRP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26460-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26460-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26460-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26460-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 26460-1-AP (SFRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, SH-SY5Y cells, U2OS cells, mouse testis tissue
• Positive IHC detected in: human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

26460-1-AP targets SFRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRP1 fusion protein Ag24844
• Full Name: secreted frizzled-related protein 1
• Calculated Molecular Weight: 314 aa, 35 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC036503
• Gene Symbol: SFRP1
• Gene ID (NCBI): 6422
• RRID: AB_2880522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	APL Bioeng; Oscillatory shear stress-induced downregulation of TET1s injures vascular endothelial planar cell polarity by suppression of actin polymerization; Authors - Kai Qu ; KD Validated
human	WB	Aging (Albany NY); Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.; Authors - Liansheng Sha
rat	IHC	Cells; Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells.; Authors - Keita Ipposhi
mouse	WB	Stem Cell Rev Rep; Interleukin-19 in Bone Marrow Contributes to Bone Loss Via Suppressing Osteogenic Differentiation Potential of BMSCs in Old Mice; Authors - Enjun He
human,mouse	WB	In Vitro Cell Dev Biol Anim; SFRP1 reduces neutrophil infiltration and inhibits the Wnt/β-catenin pathway to alleviate oral submucous fibrosis; Authors - Rong Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (03-14-2023)

There are doubts about if this antibody is working in WB. There are some non-specific bands and the supposed band is very weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2, mouse liver, mouse kidney, mouse brain

SFRP1 Antibody Western Blot validation (1:2000 dilution) in HepG2, mouse liver, mouse kidney, mouse brain (Cat no:26460-1-AP)