SF4 Polyclonal antibody

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  WB analysis of HEK-293 using 15614-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15614-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15614-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 15614-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15614-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15614-1-AP

  Immunofluorescent analysis of () fixed HepG2 cells using 15614-1-AP (SF4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

SF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15614-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SURP and G-patch domain-containing protein 1, SUGP1, Splicing factor 4, RNA-binding protein RBP, F23858

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15614-1-AP targets SF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SF4 fusion protein Ag8006
• Full Name: splicing factor 4
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 55 kDa, 72 kDa
• GenBank Accession Number: BC002986
• Gene Symbol: SF4
• Gene ID (NCBI): 57794
• RRID: AB_10859818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWZ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF4 (Splicing factor 4), also known as SUGP1 (SURP and G-patch domain-containing protein 1) was first identified as a putative splicing factor based on domain composition analysis. SUGP1 protein is predicted to contain two SURP motifs, domains of alternative splicing regulators thought to be involved in RNA binding, as well as a G-patch domain, a region of six highly conserved glycine residues commonly found in eukaryotic RNA-processing proteins (PMID: 27206982). Research has shown that SUGP1 is involved in the regulation of cholesterol metabolism (PMID: 31474574).