SF4 Polyclonal antibody

SF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15614-1-AP

Synonyms

SURP and G-patch domain-containing protein 1, SUGP1, Splicing factor 4, RNA-binding protein RBP, F23858

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Validation Data Gallery

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  WB analysis of HEK-293 using 15614-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15614-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15614-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 15614-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15614-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15614-1-AP

  Immunofluorescent analysis of () fixed HepG2 cells using 15614-1-AP (SF4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15614-1-AP targets SF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SF4 fusion protein Ag8006
• Full Name: splicing factor 4
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC002986
• Gene Symbol: SF4
• Gene ID (NCBI): 57794
• RRID: AB_10859818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.