SF3B3 Monoclonal antibody, PBS Only

SF3B3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H7F5

Cat no : 67469-1-PBS

Synonyms

SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit, KIAA0017

---

Validation Data Gallery

• 

  WB analysis using 67469-1-Ig (same clone as 67469-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67469-1-Ig (SF3B3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat heart using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using SF3B3 antibody (67469-1-Ig, Clone: 2H7F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using SF3B3 antibody (67469-1-Ig, Clone: 2H7F5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

---

Product Information

67469-1-PBS targets SF3B3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SF3B3 fusion protein Ag6315
• Full Name: splicing factor 3b, subunit 3, 130kDa
• Calculated Molecular Weight: 136 kDa
• Observed Molecular Weight: 130-135 kDa
• GenBank Accession Number: BC003146
• Gene Symbol: SF3B3
• Gene ID (NCBI): 23450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.