SF3B3 Polyclonal antibody

SF3B3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 14577-1-AP

Synonyms

SF3b130, SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit

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Validation Data Gallery

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  WB analysis using 14577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14577-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14577-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 14577-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14577-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 14577-1-AP

  IP result of anti-SF3B3 (IP:14577-1-AP, 4ug; Detection:14577-1-AP 1:5000) with rat brain tissue lysate 4800ug.

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  IHC staining of human gliomas using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14577-1-AP (SF3B3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14577-1-AP (SF3B3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of mouse heart using 14577-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 14577-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 14577-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14577-1-AP

  Immunofluorescent analysis of Hela cells, using SF3B3 antibody 14577-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse heart tissue, human brain tissue, rat brain tissue, rat heart tissue, HeLa cells
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human lung cancer tissue, human gliomas tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

14577-1-AP targets SF3B3 in WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SF3B3 fusion protein Ag5980
• Full Name: splicing factor 3b, subunit 3, 130kDa
• Calculated Molecular Weight: 136 kDa
• Observed Molecular Weight: 130-135 kDa
• GenBank Accession Number: BC003146
• Gene Symbol: SF3B3
• Gene ID (NCBI): 23450
• RRID: AB_2270189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.

Publications

Species	Application	Title
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
		Genome Res; RNA structure replaces the need for U2AF2 in splicing.; Authors - Chien-Ling Lin
human	WB	Nat Commun; Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.; Authors - Teng Teng
human	RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
human	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
human	WB	Nucleic Acids Res; Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.; Authors - Ruibing Chen