SF3B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SF3B1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 27684-1-AP

Synonyms

PRP10, PRPF10, SAP 155, SAP155, SF3B1, SF3b155, Splicing factor 3B subunit 1

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Validation Data Gallery

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  WB analysis of MCF-7 using 27684-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27684-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 27684-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27684-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27684-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27684-1-AP (SF3B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27684-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27684-1-AP (SF3B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, U2OS cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

27684-1-AP targets SF3B1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SF3B1 fusion protein Ag26689
• Full Name: splicing factor 3b, subunit 1, 155kDa
• Calculated Molecular Weight: 146 kDa
• Observed Molecular Weight: 146-150 kDa
• GenBank Accession Number: NM_012433
• Gene Symbol: SF3B1
• Gene ID (NCBI): 23451
• RRID: AB_2880946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Publications

Species	Application	Title
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
human	WB	EMBO Rep; A splicing factor switch controls hematopoietic lineage specification of pluripotent stem cells.; Authors - Yapu Li ; KD Validated
human	WB	Sci Rep; Exploring the mechanistic link between SF3B1 mutation and ring sideroblast formation in myelodysplastic syndrome; Authors - Tetsuro Ochi
human	WB	Nat Commun; Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12; Authors - Zixiang Wang
human	WB	EMBO J; A transcription-independent mechanism determines rapid periodic fluctuations of BRCA1 expression; Authors - Chen Nie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wael (Verified Customer) (09-29-2022)

specific band between 100 and 150 kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hek293

SF3B1 Antibody Western Blot validation (1:2000 dilution) in Hek293 (Cat no:27684-1-AP)