SETDB2 Polyclonal antibody

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  WB analysis of Jurkat using 14428-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14428-1-AP (SETDB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SETDB2 Polyclonal Antibody for WB, ELISA

Cat No. 14428-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

C13orf4, CLLD8, CLLL8, KMT1F, Lysine N methyltransferase 1F, SET domain bifurcated 2, SET domain, bifurcated 2, SETDB2

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

14428-1-AP targets SETDB2 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SETDB2 fusion protein Ag5795
• Full Name: SET domain, bifurcated 2
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC047434
• Gene Symbol: SETDB2
• Gene ID (NCBI): 83852
• RRID: AB_2878056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96T68
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Front Oncol; Identifying key transcription factors and immune infiltration in non-small-cell lung cancer using weighted correlation network and Cox regression analyses; Authors - Jingyao Zhang ; KD Validated
human	WB	Mol Cell; DNA hypomethylation promotes UHRF1-and SUV39H1/H2-dependent crosstalk between H3K18ub and H3K9me3 to reinforce heterochromatin states; Authors - Yanqing Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shuqing (Verified Customer) (12-17-2021)

I got very nice result using this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human colon cancer cell

SETDB2 Antibody Western Blot validation (1:200 dilution) in human colon cancer cell (Cat no:14428-1-AP)