SETDB1 Recombinant antibody

SETDB1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240805D7

Cat no : 83772-5-RR

Synonyms

Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366

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Validation Data Gallery

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  WB analysis using 83772-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83772-5-RR (SETDB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83772-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83772-5-RR against Human SETDB1 were performed. The affinity constant is 32 pM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83772-5-RR is SETDB1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SETDB1 fusion protein Ag1725
• Full Name: SET domain, bifurcated 1
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: BC009362
• Gene Symbol: SETDB1
• Gene ID (NCBI): 9869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).