SETD7 Monoclonal antibody, PBS Only

SETD7 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1A9

Cat no : 68569-1-PBS

Synonyms

FLJ21193, H3 K4 HMTase SETD7, KIAA1717, KMT7, Lysine N methyltransferase 7, SET7, SET7/9, SET9, SETD7

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Validation Data Gallery

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  WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.

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Product Information

68569-1-PBS targets SETD7 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SETD7 fusion protein Ag33803
• Full Name: SET domain containing (lysine methyltransferase) 7
• Calculated Molecular Weight: 366 aa, 41 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC121055
• Gene Symbol: SETD7
• Gene ID (NCBI): 80854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).