SETD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SETD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 55377-1-AP

Synonyms

HIF 1, HIF1, HIP 1, hSET2, HSPC069, Huntingtin yeast partner B, HYPB, KIAA1732, KMT3A, Lysine N methyltransferase 3A, p231HBP, SET domain containing 2, SET2, SETD2

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Validation Data Gallery

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  WB analysis using 55377-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55377-1-AP (SETD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS cells using 55377-1-AP

  WB result of SETD2 antibody (55377-1-AP, 1:500) with siRNA transfected U2-OS cells.

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  WB analysis of Jurkat using 55377-1-AP

  WB result of SETD2 antibody (55377-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and SETD2 knockout Jurkat cells.

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  IHC staining of human colon cancer using 55377-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 55377-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55377-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD2 antibody (55377-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, U2OS cells, K-562 cells
• Positive IHC detected in: human colon cancer tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

55377-1-AP targets SETD2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SET domain containing 2
• Calculated Molecular Weight: 288 kDa
• Observed Molecular Weight: 287 kDa
• GenBank Accession Number: NM_014159
• Gene Symbol: SETD2
• Gene ID (NCBI): 29072
• RRID: AB_2881318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is a histone methyltransferase that methylates 'Lys-36' of histone H3. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters.(PMID:16118227) It is a potential tumor suppressor gene in breast cancer.(PMID:20944102) This antibody is specific to SETD2.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis.; Authors - Ruiqiong Liu ; KD Validated
human,mouse	WB,IHC	Cell Death Dis; Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC; Authors - Wei Xue ; KD Validated
human		Carcinogenesis; Proteomic analysis of the OGT interactome: novel links to epithelial-mesenchymal transition and metastasis of cervical cancer.; Authors - Jie Gao
human	WB	J Exp Clin Cancer Res; A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression.; Authors - Wentao Liu
human	CoIP,WB	Function (Oxf); Structure and Function of RhoBTB1 Required for Substrate Specificity and Cullin-3 Ubiquitination; Authors - Gaurav Kumar