SETD1A Monoclonal antibody

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• KD/KO Validated

SETD1A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1C2G12

Cat no : 67936-1-Ig

Synonyms

hSET1A, KIAA0339, KMT2F, Lysine N methyltransferase 2F, SET domain containing 1A, Set1, Set1A, SETD1A

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Validation Data Gallery

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  WB analysis using 67936-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67936-1-Ig

  WB result of SETD1A antibody (67936-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SETD1A transfected HEK-293 cells.

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  WB analysis of mouse brain using 67936-1-Ig

  Mouse brain tissue was subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at a dilution of 1:3000 and incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 67936-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67936-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67936-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67936-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

67936-1-Ig targets SETD1A in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SETD1A fusion protein Ag30424
• Full Name: SET domain containing 1A
• Calculated Molecular Weight: 186 kDa
• Observed Molecular Weight: 270 kDa
• GenBank Accession Number: NM_014712
• Gene Symbol: SETD1A
• Gene ID (NCBI): 9739
• RRID: AB_2918688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).

Publications

Species	Application	Title
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human,mouse	WB	Nat Commun; Early-life exercise induces immunometabolic epigenetic modification enhancing anti-inflammatory immunity in middle-aged male mice; Authors - Nini Zhang
human	IF	J Inflamm (Lond); Histone H3 posttranslational modified enzymes defined neutrophil plasticity and their vulnerability to IL-10 in the course of the inflammation; Authors - Paweł Piatek
human	WB	Cancer Lett; PARP1 interacts with WDR5 to enhance target gene recognition and facilitate tumorigenesis; Authors - Yali Qin
human	WB	Signal Transduct Target Ther; CXXC finger protein 1 (CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma; Authors - Tao Fan