SETBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SETBP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16841-1-AP

Synonyms

KIAA0437, SEB, SET binding protein, SET binding protein 1, SETBP1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 16841-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16841-1-AP (SETBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

16841-1-AP targets SETBP1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SETBP1 fusion protein Ag10532
• Full Name: SET binding protein 1
• Calculated Molecular Weight: 175 kDa, 26 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: BC062338
• Gene Symbol: SETBP1
• Gene ID (NCBI): 26040
• RRID: AB_2185750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETBP1 is initially found to interact with SET, which is a small protein inhibitor for tumor suppressors PP2A and NM23-H1. There are existing two isoforms of SETBP1 and the molecular weight of isoform one is 170 kDa and another is 26 kDa. SET is fused with another gene CAN through chromosome translocation in a case of acute undifferentiated leukemia. SETBP1 may involve in chromosome translocation in a case of acute T-cell leukemia [PMID:17569777]. SETBP1 can cooperate with BCR/ABL to transform committed myeloid progenitors normally lacking self-renewal capability into LSCs and cause development of myeloid leukemias resembling human CML myeloid blast crisis [PMID:22566606]. SETBP1 was shown to form a complex with SET and PP2A, enhancing the stability of SET and its inhibition of PP2A.

Publications

Species	Application	Title
human	WB	Nat Genet; Somatic SETBP1 mutations in myeloid malignancies.; Authors - Makishima Hideki H
mouse	WB	Blood; Setbp1 promotes the self-renewal of murine myeloid progenitors via activation of Hoxa9 and Hoxa10.; Authors - Oakley Kevin K ; KD Validated
human	WB,IF,IP	iScience; Recruitment of MLL1 complex is essential for SETBP1 to induce myeloid transformation.; Authors - Nhu Nguyen
human,mouse,canine	WB	J Biochem; The protein level of the tumour-promoting factor SET is regulated by cell density.; Authors - Naoki Kohyanagi ; KD Validated
human	WB	Oncotarget; Myb expression is critical for myeloid leukemia development induced by Setbp1 activation.; Authors - Nhu Nguyen