SET/TAF-I Polyclonal antibody

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  WB analysis using 55201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55201-1-AP (SET/TAF-I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55201-1-AP (SET/TAF-I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 55201-1-AP (SET/TAF-I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 55201-1-AP (SET/TAF-I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55201-1-AP (SET Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55201-1-AP (SET Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55201-1-AP (SET antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55201-1-AP (SET antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55201-1-AP (SET antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55201-1-AP (SET antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

SET/TAF-I Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55201-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

2PP2A, HLA DR associated protein II, I 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET, SET, SET nuclear oncogene, SET/TAF-I, TAF I, TAF IBETA, Template activating factor I

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: mouse spleen tissue, human kidney tissue, human liver cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

55201-1-AP targets SET/TAF-I in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SET nuclear oncogene
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 41 kDa, 39 kDa
• GenBank Accession Number: NM_003011
• Gene Symbol: SET
• Gene ID (NCBI): 6418
• RRID: AB_10837360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01105
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SET, also named as IGAAD, I-2PP2A, TAF-I, IGAAD and PHAPII, belongs to the nucleosome assembly protein (NAP) family.SET is a multifunctional oncoprotein which is ubiquitously expressed in all kinds of cells. The SET protein participates in many cellular processes including cell cycle, cell migration, apoptosis, transcription, and DNA repair. The SET protein plays important roles in facilitating cell growth and proliferation via many pathways that promote tumorigenesis and metastasis. The SET protein shows apparent molecular masses of 41 and 39 kDa (PMID: 12759364, PMID: 7753797).

Publications

Species	Application	Title
human	WB	Cell Rep; A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression; Authors - Ling Zhang ; KO Validated
human	WB	Oncotarget; Overexpression of PP2A inhibitor SET oncoprotein is associated with tumor progression and poor prognosis in human non-small cell lung cancer.; Authors - Hao Liu ; KD Validated
human	WB	J Med Chem; Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.; Authors - Brianna D Mackie
human	WB	Cancers (Basel); Pre-Clinical Study Evaluating Novel Protein Phosphatase 2A Activators as Therapeutics for Neuroblastoma.; Authors - Laura V Bownes
human	WB	Cell Signal; PMID: 25152373; Authors - Hao Liu
human	WB,IHC	BMC Cancer; MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer.; Authors - Hongli Pan