Sestrin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Sestrin 2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 21346-1-AP

Synonyms

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

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Validation Data Gallery

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  WB analysis of mouse thymus using 21346-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21346-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21346-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21346-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21346-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 21346-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Sestrin 2 antibody (21346-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse thymus tissue, HeLa cells, K-562 cells, mouse lung tissue, NIH/3T3 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 5 publications below

Product Information

21346-1-AP targets Sestrin 2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sestrin 2 fusion protein Ag15669
• Full Name: sestrin 2
• Calculated Molecular Weight: 480 aa, 54 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: NM_031459
• Gene Symbol: SESN2/Sestrin 2
• Gene ID (NCBI): 83667
• RRID: AB_10733238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Publications

Species	Application	Title
human	WB	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo
human	WB	Elife; Dynamics of mTORC1 activation in response to amino acids.; Authors - Maria Manifava ; KD Validated
mouse	WB,IF	Front Immunol; Sestrin2 provides cerebral protection through activation of Nrf2 signaling in microglia following subarachnoid hemorrhage; Authors - Youqing Yang
human	WB,IF	J Invest Dermatol; Metastatic melanoma cells rely on Sestrin2 to acquire anoikis resistance via detoxifying intracellular ROS.; Authors - Guannan Zhu ; KD Validated
rat	WB	Mol Cell Biol; Sestrin2 protects dopaminergic cells against rotenone toxicity through AMPK-dependent autophagy activation.; Authors - Yi-Sheng Hou
rat	IF	J Cereb Blood Flow Metab; Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups.; Authors - Xudan Shi