HSP47/SERPINH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSP47/SERPINH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (3)

Cat no : 10875-1-AP

Synonyms

HSP47, SERPINH1, Cell proliferation-inducing gene 14 protein, CBP2, CBP1

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Validation Data Gallery

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  WB analysis using 10875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10875-1-AP

  WB result of HSP47 antibody (10875-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47 transfected HeLa cells.

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  WB analysis of HT-1080 using 10875-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10875-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10875-1-AP (HSP47 antibody at dilution of 1:50.

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  IF Staining of HeLa using 10875-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (10875-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10875-1-AP

  Immunofluorescent analysis of HeLa cells using 10875-1-AP (HSP47 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10875-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HSP47 (10875-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HT-1080 cells, A2780 cells, A431 cells, HeLa cells, NIH/3T3 cells
• Positive IHC detected in: human stomach cancer tissue, human colon tissue, human hepatocirrhosis tissue, human lung cancer tissue, mouse kidney tissue, mouse lung tissue, rat kidney tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10875-1-AP targets HSP47/SERPINH1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSP47/SERPINH1 fusion protein Ag1301
• Full Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC014623
• Gene Symbol: HSP47/SERPINH1
• Gene ID (NCBI): 871
• RRID: AB_2301500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. The Serpin Family H Member 1 (SERPINH1) gene encodes a protein called HSP47, which is required for the correct folding and secretion of collagen (PMID: 30128672). SERPINH1 has been implicated in several collagen-related diseases, such as, brittle bone disease, keloid scars, and lung fibrosis (PMID: 32091407). SERPINH1 also correlates with the grade of glioma, and regulates proliferation, migration and invasion of glioma cell lines (PMID: 24595468). SERPINH1 regulates the Wnt/β-catenin signaling pathway and modulates gastric cancer (GC) progression (PMID: 32091407). SERPINH1 is aberrantly expressed in cervical cancer, breast cancer, glioblastoma and colorectal cancer (PMID: 32091407). SERPINH1 promotes invasion and metastasis of breast cancer cells by regulating the expression of several extracellular matrix (ECM) proteins (PMID: 25744716). Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.

Publications

Species	Application	Title
human	WB	Nat Protoc; Responsive and activable nanomedicines for remodeling the tumor microenvironment.; Authors - Yinlong Zhang ; KD Validated
human	WB,IF,IHC	Nat Commun; Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.; Authors - Xuexiang Han ; KD Validated
human,mouse	WB,IHC	Cancer Res; RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.; Authors - Nicholas Rooney
human	IP	mSystems; Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.; Authors - Xiaoqing Liu
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
mouse	WB	Sci Rep; Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes; Authors - Masahiko Terajima

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gohar (Verified Customer) (02-06-2020)

na

• Applications: Immunohistochemistry,

FH

Gohar (Verified Customer) (02-06-2020)

na

• Applications: Immunohistochemistry,

FH

Danielle (Verified Customer) (09-09-2019)

This antibody, as well as the other 5 purchased for our purposes, was sufficient and provided clear results for our experiment.

• Applications: ChIP,