Maspin Monoclonal antibody

Maspin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G5A3

Cat no : 67633-1-Ig

Synonyms

maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5

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Validation Data Gallery

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  WB analysis using 67633-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67633-1-Ig (Maspin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUMAN saliva using 67633-1-Ig

  Human saliva were subjected to SDS PAGE followed by western blot with 67633-1-Ig (Maspin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 67633-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 67633-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 67633-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Maspin antibody (67633-1-Ig, Clone: 3G5A3 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: HepG2 cells, Human saliva, HeLa cells, A431 cells
• Positive IHC detected in: human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67633-1-Ig targets Maspin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Maspin fusion protein Ag19129
• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 5
• Calculated Molecular Weight: 375 aa, 42 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC020713
• Gene Symbol: Maspin
• Gene ID (NCBI): 5268
• RRID: AB_2882834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.