SERPINB3/4 Polyclonal antibody

• Featured Product
• KD/KO Validated

SERPINB3/4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26558-1-AP

Synonyms

SERPINB3, Serpin B3, SCCA-1, Protein T4-A, Protein T4 A

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Validation Data Gallery

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  WB analysis using 26558-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 26558-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 26558-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 26558-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 26558-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human saliva tissue, L02 cells, HepG2 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

26558-1-AP targets SERPINB3/4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERPINB3/4 fusion protein Ag24846
• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 3
• Calculated Molecular Weight: 390 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC005224
• Gene Symbol: SERPINB3
• Gene ID (NCBI): 6317
• RRID: AB_2880552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4.

Publications

Species	Application	Title
human	WB	Otol Neurotol; Autophagy Is Deficient and May be Negatively Regulated by SERPINB3 in Middle Ear Cholesteatoma.; Authors - Kuen-Yao Ho
human	WB,IF	J Exp Clin Cancer Res; Super-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc.; Authors - Erbao Bian ; KD Validated
human	WB,IHC,IF	Adv Sci (Weinh); HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway; Authors - Zixian Huang ; KD Validated