Protein C inhibitor Polyclonal antibody

Protein C inhibitor Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10673-1-AP

Synonyms

Serpin A5, PROCI, Plasminogen activator inhibitor 3, Plasma serine protease inhibitor, PLANH3

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Validation Data Gallery

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  WB analysis of HepG2 using 10673-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10673-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10673-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10673-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10673-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10673-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10673-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10673-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, PC-3 cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:600
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 5 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

10673-1-AP targets Protein C inhibitor in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Protein C inhibitor fusion protein Ag1078
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC008915
• Gene Symbol: SERPINA5
• Gene ID (NCBI): 5104
• RRID: AB_2185741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.

Publications

Species	Application	Title
human	IHC	Mod Pathol; Loss of SerpinA5 protein expression is associated with advanced-stage serous ovarian tumors.; Authors - Bijsmans Ingrid T G W IT
human	IHC	J Neuropathol Exp Neurol; Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes.; Authors - Rodriguez Fausto J FJ
rat	IHC	Proteomics Clin Appl; Proteomic analysis of human articular cartilage unravels the dyscoagulation in osteoarthritis and the potential value of serpinA5 as a biomarker for osteoarthritis.; Authors - Tengjiao Zhu
human	WB,CoIP	J Cell Mol Med; SERPINA5 promotes tumour cell proliferation by modulating the PI3K/AKT/mTOR signalling pathway in gastric cancer; Authors - Meiyang Fan
rat	ELISA,IHC	Biomolecules; Improvement in Clinical Features of L-NAME-Induced Preeclampsia-like Rats through Reduced SERPINA5 Expression; Authors - Shanshui Zeng
human,rat	IHC	J Proteome Res; Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis; Authors - Qizhao Tan