SERPINA10 Monoclonal antibody

SERPINA10 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

5B3C9

Cat no : 60113-1-Ig

Synonyms

UNQ707/PRO1358, Serpin A10, PZI, PZ-dependent protease inhibitor, Protein Z-dependent protease inhibitor



Tested Applications

Positive WB detected inHepG2 cells, L02 cells, pig liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1500-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

60113-1-Ig targets SERPINA10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SERPINA10 fusion protein Ag2420
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
Calculated Molecular Weight 444 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC022261
Gene Symbol SERPINA10
Gene ID (NCBI) 51156
RRIDAB_2301526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA10 belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis.

Protocols

Product Specific Protocols
WB protocol for SERPINA10 antibody 60113-1-IgDownload protocol
IHC protocol for SERPINA10 antibody 60113-1-IgDownload protocol
IF protocol for SERPINA10 antibody 60113-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis.

Authors - Wenwen Guo