SERF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SERF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11691-1-AP

Synonyms

small EDRK rich factor 2, Protein 4F5-related, Protein 4F5 related, h4F5rel, Gastric cancer-related protein VRG107

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Validation Data Gallery

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  WB analysis of HepG2 using 11691-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11691-1-AP (SERF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11691-1-AP

  IP result of anti-SERF2 (IP:11691-1-AP, 4ug; Detection:11691-1-AP 1:500) with HepG2 cells lysate 4000ug.

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  IHC staining of human skin cancer using 11691-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 11691-1-AP (SERF2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 11691-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SERF2 antibody (11691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 11691-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SERF2 antibody (11691-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

11691-1-AP targets SERF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERF2 fusion protein Ag2305
• Full Name: small EDRK-rich factor 2
• Calculated Molecular Weight: 59 aa, 7 kDa
• GenBank Accession Number: BC022326
• Gene Symbol: SERF2
• Gene ID (NCBI): 10169
• RRID: AB_10596937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERF2 Belongs to the SERF family. The antibody is specific to SERF2.

Publications

Species	Application	Title
mouse	WB	Mamm Genome; A novel knockout mouse for the small EDRK-rich factor 2 (Serf2) showing developmental and other deficits.; Authors - Karen Cleverley ; KD Validated
mouse	WB	Life Sci Alliance; Deletion of SERF2 in mice delays embryonic development and alters amyloid deposit structure in the brain; Authors - Esther Stroo