CoraLite® Plus 488-conjugated SERCA2,ATP2A2 Monoclonal antibody

SERCA2,ATP2A2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F11A11

Cat no : CL488-67248

Synonyms

ATP2A2, SERCA2, SERCA2b, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Calcium pump 2

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Validation Data Gallery

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  FC experiment of HeLa using CL488-67248

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SERCA2,ATP2A2 (CL488-67248, Clone:1F11A11) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67248 targets SERCA2,ATP2A2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SERCA2,ATP2A2 fusion protein Ag5052
• Full Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Calculated Molecular Weight: 1042 aa, 115 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC035588
• Gene Symbol: SERCA2
• Gene ID (NCBI): 488
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C