Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human urothelial carcinoma tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
67248-1-Ig targets SERCA2,ATP2A2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SERCA2,ATP2A2 fusion protein Ag5052 Predict reactive species |
| Full Name | ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 |
| Calculated Molecular Weight | 1042 aa, 115 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC035588 |
| Gene Symbol | SERCA2 |
| Gene ID (NCBI) | 488 |
| RRID | AB_2882525 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P16615 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SERCA2,ATP2A2 antibody 67248-1-Ig | Download protocol |
| IHC protocol for SERCA2,ATP2A2 antibody 67248-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Physiol microRNA-151-3p regulates slow muscle gene expression by targeting ATP2a2 in skeletal muscle cells. | ||
Biomolecules Urolithin A Protects Hepatocytes from Palmitic Acid-Induced ER Stress by Regulating Calcium Homeostasis in the MAM | ||
Cell Calcium Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release. | ||
Ecotoxicol Environ Saf Fat mass and obesity-associated gene (FTO) hypermethylation induced by decabromodiphenyl ethane causing cardiac dysfunction via glucolipid metabolism disorder. | ||
Am J Physiol Gastrointest Liver Physiol EMC3 is critical for CFTR function and calcium mobilization in the mouse intestinal epithelium | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Susmita (Verified Customer) (06-13-2022) | This antibody is very good for WB analysis.
|
FH Iram (Verified Customer) (03-11-2022) | Very good antibody
|
