SERCA2,ATP2A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SERCA2,ATP2A2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 27311-1-AP

Synonyms

ATP2A2, SERCA2, SERCA2b, ATP2A 2, DAR

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Validation Data Gallery

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  WB analysis using 27311-1-AP

  Boiled and unboiled mouse heart lysates were subjected to SDS PAGE followed by western blot with 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27311-1-AP

  Boiled and 37°C incubated HEK-293 cells lysates were subjected to SDS PAGE followed by western blot with 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HEK-293 cells, mouse heart unboiled, 37°C incubated HEK-293 cells
• Positive IHC detected in: mouse skeletal muscle tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue
• Positive IF-Fro detected in: mouse heart tissue
• Positive IF/ICC detected in: C2C12 cells, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

27311-1-AP targets SERCA2,ATP2A2 in WB, IHC, IF/ICC, IF-P, IF-Fro, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERCA2,ATP2A2 fusion protein Ag26177
• Full Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Calculated Molecular Weight: 1042 aa, 115 kDa
• Observed Molecular Weight: 110-115 kDa
• GenBank Accession Number: BC035588
• Gene Symbol: SERCA2
• Gene ID (NCBI): 488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

Publications

Species	Application	Title
mouse	WB	JCI Insight; Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.; Authors - Yingchao Shi
human	WB,CoIP	Nat Commun; ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndrome; Authors - Mailis Liiv
human	WB	Biochem Biophys Res Commun; Mitigation of ROS-triggered endoplasmic reticulum stress by upregulating Nrf2 retards diabetic nephropathy; Authors - Xiaojiao Zeng
human	WB,IHC	FASEB J; Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis; Authors - Xiang Gao