SERBP1 Monoclonal antibody

SERBP1 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig, Canine

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1D4H1

Cat no : 67792-1-Ig

Synonyms

CGI 55, CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI RBP1, PAI1 RNA binding protein 1, PAIRBP1, SERBP1

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Validation Data Gallery

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  WB analysis using 67792-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of MDCK using 67792-1-Ig

  MDCK cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67792-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 67792-1-Ig

  IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug.

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  IP experiment of HeLa using 67792-1-Ig

  IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug.

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  IHC staining of human liver cancer using 67792-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67792-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67792-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERBP1 antibody (67792-1-Ig, Clone: 1D4H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).

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Tested Applications

• Positive WB detected in: LNCaP cells, MDCK cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67792-1-Ig targets SERBP1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, Canine samples.

• Tested Reactivity: Human, Mouse, Rat, pig, Canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SERBP1 fusion protein Ag30202
• Full Name: SERPINE1 mRNA binding protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC008045
• Gene Symbol: SERBP1
• Gene ID (NCBI): 26135
• RRID: AB_2918556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).