Septin 5 Polyclonal antibody

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  WB analysis of Jurkat using 11631-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11631-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11631-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11631-1-AP

  IP result of anti-Septin 5 (IP:11631-1-AP, 3ug; Detection:11631-1-AP 1:300) with mouse brain tissue lysate 1000ug.

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  IHC staining of human gliomas using 11631-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11631-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Septin 5 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11631-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CDCREL, CDCREL 1, CDCREL1, H5, Peanut like protein 1, PNUTL1, SEPT5, Septin 5, septin5

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, human brain tissue, NIH/3T3 cells, rat brain tissue, K-562 cells, LNCaP cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

11631-1-AP targets Septin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Septin 5 fusion protein Ag2205
• Full Name: septin 5
• Calculated Molecular Weight: 369 aa, 43 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC025261
• Gene Symbol: Septin 5
• Gene ID (NCBI): 5413
• RRID: AB_2301692
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99719
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.

Publications

Species	Application	Title
mouse,rat	WB,IF,IP	J Neurosci; Cyclin-dependent kinase 5 phosphorylation of human septin SEPT5 (hCDCrel-1) modulates exocytosis.; Authors - Amin Niranjana D ND
mouse	WB	Neurobiol Dis; S327 phosphorylation of the presynaptic protein SEPTIN5 increases in the early stages of neurofibrillary pathology and alters the functionality of SEPTIN5.; Authors - Catarina B Ferreira
mouse	WB	Front Mol Neurosci; Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice; Authors - Xiaoxia Li
human	WB	Cell Signal; SEPT5 overexpression predicts poor prognosis and promotes progression through feedback regulation of HIF-1α in clear cell renal cell carcinoma; Authors - Wenjing Liu ; KD Validated
human	IF,WB	Cells; Presynaptic Vesicle Protein SEPTIN5 Regulates the Degradation of APP C-Terminal Fragments and the Levels of Aβ.; Authors - Mikael Marttinen ; KO Validated
rat	WB	Molecules; Synergistic Neuroprotective Effect of Endogenously-Produced Hydroxytyrosol and Synaptic Vesicle Proteins on Pheochromocytoma Cell Line Against Salsolinol.; Authors - Robina Manzoor