Septin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Septin 2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

25

Reviews (2)

Cat no : 11397-1-AP

Synonyms

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

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Validation Data Gallery

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  WB analysis using 11397-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11397-1-AP

  WB result of Septin 2 antibody (11397-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 2 transfected HEK-293 cells.

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  WB analysis of human skeletal muscle using 11397-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 11397-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11397-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 11397-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11397-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11397-1-AP

  IP result of anti-Septin 2 (IP:11397-1-AP, 3ug; Detection:11397-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

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  IHC staining of human liver cancer using 11397-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11397-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11397-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11397-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11397-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11397-1-AP (Septin 2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Septin 2 antibody (11397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of hTERT-RPE1 cells and Mouse embryonic fibroblasts using 11397-1-AP

  IF result from Dr. Kevin Corbit. Septin 2 (11397-1-AP) marks the transition zone and cilium of Human hTERT-RPE1 cells and Mouse embryonic fibroblasts.

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  FC experiment of MCF-7 using 11397-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Septin 2 (11397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, HeLa cells, human skeletal muscle tissue, rat brain tissue, C6 cells, HEK-293 cells, K-562 cells, Jurkat cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue, human colon tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, hTERT-RPE1 cells and Mouse embryonic fibroblasts
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Product Information

11397-1-AP targets Septin 2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Septin 2 fusion protein Ag1946
• Full Name: septin 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC014455
• Gene Symbol: Septin 2
• Gene ID (NCBI): 4735
• RRID: AB_2239110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEPT2 belongs to a family of septins, GTP-binding proteins that are a component of the cytoskeleton. Septins can assemble into intracellular structures of various shapes and are known to influence membrane stability, forming diffusion barriers and serving as scaffolds.

What is the molecular weight of SEPT2? Is SEPT2 post-translationally modified?

The molecular weight of SEPT2 is 45 kDa. In a cellular context, SEPT2 forms hetero-hexamers and hetero-octamers with other septins. These hexamers and octamers further assemble into high-order structures such as filaments and rings. SEPT2 can be phosphorylated on Ser218 by casein kinase 2 (PMID: 19165576) and can also be SUMOylated (PMID: 29051266).

What is the subcellular localization of SEPT2?

SEPT2 forms a diffusion barrier and localizes to the primary cilium; in some cell types, it has been found at the transition zone/basal body (PMID: 20558667), while in others localized to the axoneme (PMID: 23572511). During cell division, SEPT2 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody (PMID: 9203580). In interphase cells, SEPT2 was found to associate with polyglutamylated microtubules (PMID: 18209106) and can affect microtubule dynamics via interaction with MAP4.

What is the tissue expression pattern of SEPT2?

While some septins have tissue-specific expression profiles, SEPT2 is ubiquitously expressed (PMID: 22314400).

Publications

Species	Application	Title
human	IF	Nat Commun; Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells.; Authors - Pamela P Lee
human	WB,IF	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
mouse	WB,IF	PLoS Genet; Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.; Authors - Manoj B Menon
human		Cell Death Dis; JMJD2C-mediated long non-coding RNA MALAT1/microRNA-503-5p/SEPT2 axis worsens non-small cell lung cancer.; Authors - Jun Zhang
mouse	WB	Elife; Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.; Authors - Julia Patzig
mouse	IF	PLoS Genet; SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.; Authors - Yi-Ru Shen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Incubation for a few hours at room temperature on HEK293T lysates. Detection of a very strong signal just below the 47 kDa marker (expected size of Septin 2) and a fainter signal just above the 47 kDa marker.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Mohammed (Verified Customer) (08-19-2020)

Some staining at the annulus, there seems to be staining in the acrosome. Overall, really high background (even when the concentration is lowered).

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis