SENP1 Recombinant antibody

SENP1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240478D3

Cat no : 83594-4-RR

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3

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Validation Data Gallery

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  WB analysis using 83594-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovarian cancer using 83594-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83594-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83594-4-RR against Human SENP1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HL-60 cells, Jurkat cells, rat testis tiuuse
• Positive IHC detected in: human ovarian cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83594-4-RR is SENP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SENP1 fusion protein Ag18028
• Full Name: SUMO1/sentrin specific peptidase 1
• Calculated Molecular Weight: 644 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC045639
• Gene Symbol: SENP1
• Gene ID (NCBI): 29843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.