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  • KD/KO Validated

SENP1 Polyclonal antibody

SENP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 25349-1-AP

Synonyms

SENP1, Sentrin specific protease 1, SuPr 2, SuPr2, SENP 1



Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse testis tissue, rat testis tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman ovary tissue, human liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25349-1-AP targets SENP1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SENP1 fusion protein Ag18028
Full Name SUMO1/sentrin specific peptidase 1
Calculated Molecular Weight 644 aa, 73 kDa
Observed Molecular Weight 73-74 kDa
GenBank Accession NumberBC045639
Gene Symbol SENP1
Gene ID (NCBI) 29843
RRIDAB_2880039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

Protocols

Product Specific Protocols
WB protocol for SENP1 antibody 25349-1-APDownload protocol
IHC protocol for SENP1 antibody 25349-1-APDownload protocol
IP protocol for SENP1 antibody 25349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Lactate regulates cell cycle by remodeling the anaphase promoting complex

Authors - Weihai Liu
  • KD Validated
human,mouseWB

Free Radic Biol Med

Sentrin-specific protease 1 maintains mitochondrial homeostasis through targeting the deSUMOylation of sirtuin-3 to alleviate oxidative damage induced by hepatic ischemia/reperfusion

Authors - Kang Xia
humanWB

Int Immunopharmacol

Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma

Authors - Jiayuan Liang
humanWB, IHC

Cancer Med

Long noncoding RNA MCM3AP-AS1 enhances cell proliferation and metastasis in colorectal cancer by regulating miR-193a-5p/SENP1.

Authors - Mingyue Zhou
humanWB

J Pharmacol Sci

Momordin Ic induces G0/1 phase arrest and apoptosis in colon cancer cells by suppressing SENP1/c-MYC signaling pathway.

Authors - Fang Xianjun
  • KD Validated
WB

J Virol

Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication

Authors - Xiangju Wu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Chun (Verified Customer) (09-07-2020)

This is a great antibody for immunoblotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa