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SENP1 Polyclonal antibody
SENP1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 25349-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse testis tissue, rat testis tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human ovary tissue, human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 6 publications below |
WB | See 13 publications below |
IHC | See 2 publications below |
CoIP | See 1 publications below |
Product Information
25349-1-AP targets SENP1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SENP1 fusion protein Ag18028 |
Full Name | SUMO1/sentrin specific peptidase 1 |
Calculated Molecular Weight | 644 aa, 73 kDa |
Observed Molecular Weight | 73-74 kDa |
GenBank Accession Number | BC045639 |
Gene Symbol | SENP1 |
Gene ID (NCBI) | 29843 |
RRID | AB_2880039 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.
Protocols
Product Specific Protocols | |
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WB protocol for SENP1 antibody 25349-1-AP | Download protocol |
IHC protocol for SENP1 antibody 25349-1-AP | Download protocol |
IP protocol for SENP1 antibody 25349-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Lactate regulates cell cycle by remodeling the anaphase promoting complex
| ||
Free Radic Biol Med Sentrin-specific protease 1 maintains mitochondrial homeostasis through targeting the deSUMOylation of sirtuin-3 to alleviate oxidative damage induced by hepatic ischemia/reperfusion | ||
Int Immunopharmacol Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma | ||
Cancer Med Long noncoding RNA MCM3AP-AS1 enhances cell proliferation and metastasis in colorectal cancer by regulating miR-193a-5p/SENP1. | ||
J Pharmacol Sci Momordin Ic induces G0/1 phase arrest and apoptosis in colon cancer cells by suppressing SENP1/c-MYC signaling pathway.
| ||
J Virol Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Chun (Verified Customer) (09-07-2020) | This is a great antibody for immunoblotting.
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