SENP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SENP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 25349-1-AP

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1

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Validation Data Gallery

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  WB analysis using 25349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25349-1-AP (SENP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25349-1-AP

  IP result of anti-SENP1 (IP:25349-1-AP, 4ug; Detection:25349-1-AP 1:1000) with Jurkat cells lysate 1400 ug.

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  IHC staining of human ovary using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary cancer using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 25349-1-AP (SENP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human ovary cancer tissue, human liver tissue, human ovary tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below

Product Information

25349-1-AP targets SENP1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SENP1 fusion protein Ag18028
• Full Name: SUMO1/sentrin specific peptidase 1
• Calculated Molecular Weight: 644 aa, 73 kDa
• Observed Molecular Weight: 73-74 kDa
• GenBank Accession Number: BC045639
• Gene Symbol: SENP1
• Gene ID (NCBI): 29843
• RRID: AB_2880039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

Publications

Species	Application	Title
human	WB	Nature; Lactate regulates cell cycle by remodeling the anaphase promoting complex; Authors - Weihai Liu ; KD Validated
human,mouse	WB	Free Radic Biol Med; Sentrin-specific protease 1 maintains mitochondrial homeostasis through targeting the deSUMOylation of sirtuin-3 to alleviate oxidative damage induced by hepatic ischemia/reperfusion; Authors - Kang Xia
human	WB	Int Immunopharmacol; Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma; Authors - Jiayuan Liang
human	WB, IHC	Cancer Med; Long noncoding RNA MCM3AP-AS1 enhances cell proliferation and metastasis in colorectal cancer by regulating miR-193a-5p/SENP1.; Authors - Mingyue Zhou
human	WB	J Pharmacol Sci; Momordin Ic induces G0/1 phase arrest and apoptosis in colon cancer cells by suppressing SENP1/c-MYC signaling pathway.; Authors - Fang Xianjun ; KD Validated
	WB	J Virol; Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication; Authors - Xiangju Wu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This is a great antibody for immunoblotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa