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  • KD/KO Validated

SENP1 Polyclonal antibody

SENP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 25349-1-AP

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1



Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse testis tissue, rat testis tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman ovary cancer tissue, human liver tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25349-1-AP targets SENP1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SENP1 fusion protein Ag18028
Full Name SUMO1/sentrin specific peptidase 1
Calculated Molecular Weight 644 aa, 73 kDa
Observed Molecular Weight 73-74 kDa
GenBank Accession NumberBC045639
Gene Symbol SENP1
Gene ID (NCBI) 29843
RRIDAB_2880039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

Protocols

Product Specific Protocols
WB protocol for SENP1 antibody 25349-1-APDownload protocol
IHC protocol for SENP1 antibody 25349-1-APDownload protocol
IP protocol for SENP1 antibody 25349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Lactate regulates cell cycle by remodeling the anaphase promoting complex

Authors - Weihai Liu
  • KD Validated
human,mouseWB

Free Radic Biol Med

Sentrin-specific protease 1 maintains mitochondrial homeostasis through targeting the deSUMOylation of sirtuin-3 to alleviate oxidative damage induced by hepatic ischemia/reperfusion

Authors - Kang Xia
humanWB

Int Immunopharmacol

Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma

Authors - Jiayuan Liang
humanWB, IHC

Cancer Med

Long noncoding RNA MCM3AP-AS1 enhances cell proliferation and metastasis in colorectal cancer by regulating miR-193a-5p/SENP1.

Authors - Mingyue Zhou
humanWB

J Pharmacol Sci

Momordin Ic induces G0/1 phase arrest and apoptosis in colon cancer cells by suppressing SENP1/c-MYC signaling pathway.

Authors - Fang Xianjun
  • KD Validated
WB

J Virol

Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication

Authors - Xiangju Wu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Chun (Verified Customer) (09-07-2020)

This is a great antibody for immunoblotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa