SEMA4C Polyclonal antibody, PBS Only

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  WB analysis using 28402-1-AP (same clone as 28402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 28402-1-AP (same clone as 28402-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  WB analysis using 28402-1-AP (same clone as 28402-1-PBS)

  Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

SEMA4C Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28402-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

28402-1-PBS targets SEMA4C in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEMA4C fusion protein Ag29143
• Full Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
• Calculated Molecular Weight: 833 aa, 93 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC109104
• Gene Symbol: SEMA4C
• Gene ID (NCBI): 54910
• RRID: AB_2881134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C0C4
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SEMA4C is a member of transmembrane semaphorin proteins which regulate axonal guidance in the developing nervous system. Furthermore, studies have demonstrated that sema4C was also implicated in biological events outside the nervous system, such as cell migration, tumor development, and terminal myogenic differentiation (PMID: 36010604). SEMA4C is highly expressed in breast, lung, cervical, and liver cancers. SEMA4C is involved in oncogenic signaling and is a potential therapeutic target for invasive breast cancer, hepatocellular carcinoma, glioma, and osteosarcoma (PMID: 33177246).