Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody

SELPLG/CD162 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241297F10

Cat no : 98084-1-RR

Synonyms

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 98084-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10), or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98084-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10) (red), or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98084-1-RR

  1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98084-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98084-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.699 nM.

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98084-1-RR targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: selectin P ligand
• Calculated Molecular Weight: 412 aa, 43 kDa
• GenBank Accession Number: BC029782
• Gene Symbol: SELPLG
• Gene ID (NCBI): 6404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).