P-selectin / CD62P Monoclonal antibody, PBS Only

P-selectin / CD62P Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G2C7

Cat no : 60322-1-PBS

Synonyms

CD62P, P-selectin, GMP140, GMP 140, CD62 antigen-like family member P

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Validation Data Gallery

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  WB analysis using 60322-1-Ig (same clone as 60322-1-PBS)

  Untreated and PNGase F-treated lysates of human platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood platelets using 60322-1-Ig (same clone as 60322-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60322-1-Ig (same clone as 60322-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 60322-1-Ig (same clone as 60322-1-PBS)

  IP result of anti-P-selectin / CD62P (IP:60322-1-Ig, 5ug; Detection:60322-1-Ig 1:600) with K-562 cells lysate 1760ug. This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

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Product Information

60322-1-PBS targets P-selectin / CD62P in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: P-selectin / CD62P fusion protein Ag4124
• Full Name: selectin P (granule membrane protein 140kDa, antigen CD62)
• Calculated Molecular Weight: 830 aa, 91 kDa
• Observed Molecular Weight: 140 kDa, 105-110 kDa
• GenBank Accession Number: BC028067
• Gene Symbol: CD62P
• Gene ID (NCBI): 6403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.