SELENBP1 Polyclonal antibody

SELENBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27449-1-AP

Synonyms

Methanethiol oxidase, LPSB, hSP56, hSBP, EC:1.8.3.4

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Validation Data Gallery

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  WB analysis using 27449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 27449-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 27449-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27449-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27449-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 27449-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 27449-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27449-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue, L02 cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue, human colon tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27449-1-AP targets SELENBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SELENBP1 fusion protein Ag24453
• Full Name: selenium binding protein 1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC009084
• Gene Symbol: SELENBP1
• Gene ID (NCBI): 8991
• RRID: AB_2880872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SELENBP1 (selenium binding protein 1,SBP1), also known as LPSB or SP56, is a 472 amino acid peripheral membrane protein that binds selenium covalently. Selenium, an essential trace element, has been shown to have an anti-cancer effect. Many reports have described a relationship between insufficient selenium intake and increased risk of cancer. Moreover, the expression of SELENBP1 has been shown to be decreased in several tumors including cancers of the prostate, lungs, colon, and ovary (PMID: 21143902, 20332323, 18272210). SELENBP1 has four isoforms with the molecular mass of 56kD, 52kD and 45kD.