SEL1L Recombinant antibody, PBS Only

SEL1L Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241594G2

Cat no : 84317-7-PBS

Synonyms

Suppressor of lin-12-like protein 1, Sel-1L, Sel 1L, Protein sel-1 homolog 1, Protein sel 1 homolog 1

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Validation Data Gallery

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  WB analysis using 84317-7-RR (same clone as 84317-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84317-7-RR (SEL1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84317-7-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 84317-7-RR (same clone as 84317-7-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SEL1L Recombinant antibody (84317-7-RR, Clone:241594G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84317-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84317-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84317-7-RR against Human SEL1L were performed. The affinity constant is 6.03 nM.

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Product Information

The immunogen of 84317-7-PBS is SEL1L Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEL1L fusion protein Ag32178
• Full Name: sel-1 suppressor of lin-12-like (C. elegans)
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: NM_005065
• Gene Symbol: SEL1L
• Gene ID (NCBI): 6400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen. .