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SEC24D Monoclonal antibody, PBS Only

SEC24D Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G5G9

Cat no : 67409-1-PBS

Synonyms

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SEC24D Monoclonal antibody (67409-1-Ig)

    WB analysis of Jurkat using 67409-1-Ig (same clone as 67409-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SEC24D Monoclonal antibody (67409-1-Ig)

    WB analysis of HepG2 using 67409-1-Ig (same clone as 67409-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SEC24D Monoclonal antibody (67409-1-Ig)

    WB analysis of HeLa using 67409-1-Ig (same clone as 67409-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC24D Monoclonal antibody (67409-1-Ig)

    IF Staining of HepG2 using 67409-1-Ig (same clone as 67409-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC24D antibody (67409-1-Ig, Clone: 3G5G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

Product Information

67409-1-PBS targets SEC24D in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SEC24D fusion protein Ag4657
Full Name SEC24 family, member D (S. cerevisiae)
Calculated Molecular Weight 1033 aa, 113 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC035761
Gene Symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2882650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).