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SEC24D Polyclonal antibody, PBS Only

SEC24D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13673-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC24D Polyclonal antibody (13673-1-AP)

    WB analysis using 13673-1-AP (same clone as 13673-1-PBS)

    Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13673-1-AP (SEC24D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SEC24D Polyclonal antibody (13673-1-AP)

    IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SEC24D Polyclonal antibody (13673-1-AP)

    IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC24D Polyclonal antibody (13673-1-AP)

    IF Staining of HeLa using 13673-1-AP (same clone as 13673-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13673-1-AP (SEC24D antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

Product Information

13673-1-PBS targets SEC24D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC24D fusion protein Ag4657
Full Name SEC24 family, member D (S. cerevisiae)
Calculated Molecular Weight 1033 aa, 113 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC035761
Gene Symbol SEC24D
Gene ID (NCBI) 9871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).