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SEC24D Polyclonal antibody, PBS Only

SEC24D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13673-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC24D Polyclonal antibody (13673-1-AP)

    WB analysis using 13673-1-AP (same clone as 13673-1-PBS)

    Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13673-1-AP (SEC24D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SEC24D Polyclonal antibody (13673-1-AP)

    IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SEC24D Polyclonal antibody (13673-1-AP)

    IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC24D Polyclonal antibody (13673-1-AP)

    IF Staining of HeLa using 13673-1-AP (same clone as 13673-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13673-1-AP (SEC24D antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

Product Information

13673-1-PBS targets SEC24D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC24D fusion protein Ag4657
Full Name SEC24 family, member D (S. cerevisiae)
Calculated Molecular Weight 1033 aa, 113 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC035761
Gene Symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2877968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).