SEC24D Polyclonal antibody, PBS Only

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  WB analysis using 13673-1-AP (same clone as 13673-1-PBS)

  Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13673-1-AP (SEC24D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13673-1-AP (same clone as 13673-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13673-1-AP (SEC24D antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

SEC24D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 13673-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

13673-1-PBS targets SEC24D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC24D fusion protein Ag4657
• Full Name: SEC24 family, member D (S. cerevisiae)
• Calculated Molecular Weight: 1033 aa, 113 kDa
• Observed Molecular Weight: 113 kDa
• GenBank Accession Number: BC035761
• Gene Symbol: SEC24D
• Gene ID (NCBI): 9871
• RRID: AB_2877968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94855
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).