SEC24C Polyclonal antibody

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  WB analysis of HeLa using 16073-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16073-1-AP (SEC24C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16073-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16073-1-AP (SEC24C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 16073-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16073-1-AP (SEC24C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SEC24C Polyclonal Antibody for WB, IF, ELISA

Cat No. 16073-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

KIAA0079, SEC24 related protein C, SEC24C

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16073-1-AP targets SEC24C in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC24C fusion protein Ag9030
• Full Name: SEC24 family, member C (S. cerevisiae)
• Calculated Molecular Weight: 1094 aa, 118 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC018928
• Gene Symbol: SEC24C
• Gene ID (NCBI): 9632
• RRID: AB_2186121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53992
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).