Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 20892-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 20892-1-AP
IP result of anti-SEC23IP (IP:20892-1-AP, 4ug; Detection:20892-1-AP 1:1000) with HeLa cells lysate 3600ug.
Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 20892-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
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HEK-293 cells, mouse kidney tissue, HeLa cells, U-251 cells
Positive IP detected in
HeLa cells
Positive IHC detected in
human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SEC23-interacting protein (SEC23IP), also known as p125, is a member of the phosphatidic acid preferring-phospholipase A1 family. SEC23IP interacts with Sec23, a coat component of COPII vesicles which are involved in protein export from the endoplasmic reticulum (ER). SEC23IP localizes to ER exit sites and participates in the organization of this compartment (PMID: 15623529). It may have a role in the early stage of the secretory pathway (PMID: 10400679).
Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 20892-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human heart using 20892-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 20892-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 20892-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 20892-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of HeLa using 20892-1-AP
IP result of anti-SEC23IP (IP:20892-1-AP, 4ug; Detection:20892-1-AP 1:1000) with HeLa cells lysate 3600ug.
IF/ICC Figures
IF Staining of HeLa using 20892-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SEC23IP Polyclonal antibody
Catalog Number
20892-1-AP
Citations
2
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
