SEC23IP Polyclonal antibody

SEC23IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20892-1-AP

Synonyms

SEC23-interacting protein, p125, MSTP053

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Validation Data Gallery

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  WB analysis using 20892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20892-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20892-1-AP

  IP result of anti-SEC23IP (IP:20892-1-AP, 4ug; Detection:20892-1-AP 1:1000) with HeLa cells lysate 3600ug.

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  IHC staining of human heart using 20892-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20892-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 20892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 20892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20892-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, HeLa cells, U-251 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human heart tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

20892-1-AP targets SEC23IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC23IP fusion protein Ag14916
• Full Name: SEC23 interacting protein
• Calculated Molecular Weight: 1000 aa, 111 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC063800
• Gene Symbol: SEC23IP
• Gene ID (NCBI): 11196
• RRID: AB_10896458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC23-interacting protein (SEC23IP), also known as p125, is a member of the phosphatidic acid preferring-phospholipase A1 family. SEC23IP interacts with Sec23, a coat component of COPII vesicles which are involved in protein export from the endoplasmic reticulum (ER). SEC23IP localizes to ER exit sites and participates in the organization of this compartment (PMID: 15623529). It may have a role in the early stage of the secretory pathway (PMID: 10400679).

Publications

Species	Application	Title
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
human	IF	J Cell Biol; Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells; Authors - Miharu Maeda