SEC16A Monoclonal antibody

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  WB analysis using 68493-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68493-1-Ig (SEC16A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

SEC16A Monoclonal Antibody for WB, ELISA

Cat No. 68493-1-Ig

Clone No.1D9A5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

FLJ26737, KIAA0310, p250, RP11 413M3.10, SEC16, SEC16 homolog A, SEC16A, SEC16L

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68493-1-Ig targets SEC16A in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SEC16 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 234 kDa
• Observed Molecular Weight: 250-300 kDa
• GenBank Accession Number: NM_014866
• Gene Symbol: SEC16A
• Gene ID (NCBI): 9919
• RRID: AB_3085204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O15027
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.