SEC14L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SEC14L1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25541-1-AP

Synonyms

SEC14-like protein 1, SEC14L

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Validation Data Gallery

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  WB analysis using 25541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 25541-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 25541-1-AP

  IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:500) with HEK-293 cells lysate 1480 ug.

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  IP experiment of HEK-293 using 25541-1-AP

  IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:300) with HEK-293 cells lysate 2600ug.

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  IF Staining of A431 using 25541-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SEC14L1 antibody (25541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Y79 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

25541-1-AP targets SEC14L1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC14L1 fusion protein Ag22233
• Full Name: SEC14-like 1 (S. cerevisiae)
• Calculated Molecular Weight: 719 aa, 82 kDa
• Observed Molecular Weight: 81 kDa, 77 kDa
• GenBank Accession Number: BC142979
• Gene Symbol: SEC14L1
• Gene ID (NCBI): 6397
• RRID: AB_2880125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC14L1 (SEC14-like protein 1) is a member of SEC14 family in mammals with the potential to control local phospholipid content in membranes. SEC14L1 may act as a phospholipid transfer protein. It has also been reported that SEC14L1 functions as a novel negative regulator of RIG-I-mediated antiviral signaling and is involved in innate immunity (PMID: 17092608; 23843640). SEC14L1 has three isoforms with the molecular mass of 81.2kDa, 81.8kDa and 77.1kDa.

Publications

Species	Application	Title
human	WB	Elife; The ORP9-ORP11 dimer promotes sphingomyelin synthesis; Authors - Birol Cabukusta ; KO Validated
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi